GC-AED 3-Day Training Course Outline

Module 1: GC-AED Overview

1.1 Definitions
1.2 System Photograph
1.3 GC-AED History
1.4 Current Commercial Technology
1.5 Key Application Areas
1.6 Example Chromatogram

Module 2: Theory of Operation

2.1 Hyphenation with Gas Chromatography
2.2 Plasma Diagram and Operation
2.3 Reagent Gases
2.4 Spectrometer Design
2.5 AED Signal Generation
2.6 Table of Selected Spectral Lines

Module 3: Instrument Layout

3.1 GC-AED System Block Diagram
3.2 Plasma Emission Source and Spectrometer
3.3 Maintenance Issues and QC Check
3.4 AED Gas System Requirements

Module 4: ChemStation and Software

4.1 Software Overview
4.2 GC-AED ChemStation
4.3 AED Real-time Spectral Plot
4.4 Main AED Screen
4.5 Special Requirements for AED Data Analysis

Module 5: Methods and Parameters Overview

5.1 Elemental Analysis Overview
5.2 Evaluating the AED for a new Application
5.3 Recipes and Reagent Gases
5.4 Back Amount and Suppression
5.5 Compound-Independent Calibration
5.6 Setting the Injection Amount
5.7 Other Method and Parameter Considerations

Module 6: Initiate a GC-AED Run

6.1 Before you Start
6.2 Start the Run
6.3 Data Analysis

Module 7: Application Overview

7.1 Unknown Identification
7.2 Compound Independent Calibration (CIC )
7.3 Environmental Applications
7.4 Petroleum Applications
7.5 Liquid Petroleum Gases
7.6 Specialty Chemicals
7.7 High-tech Applications
7.8 Food and Fragrances
7.9 Organometallics
7.10 Warfare Agents and Stable Isotopes

Module 8: GC-AED Supplemental Materials and Services

8.1 GC-AED Publication List
8.2 Agilent Documents
8.3 AED Discussion List
8.4 Custom GC-AED Macros for Data Reduction
8.5 Simple User Interfaces and Automation

Module 9: Atomic Emission Detection

9.1 System Overview
9.2 Microwave and Ultraviolet Hazards
9.3 Microwave Cavity
9.4 AED Gas System Schematic
9.5 Solvent Venting Issues
9.6 GC-AED Performance Attributes
9.7 Spectrometer and Photo Diode Array
9.8 AED Signal Generation

Module 10: Real Time Display

10.1 Overview of ChemStation Real Time Displays
10.2 AED Real Time Spectral Plot
10.3 "Rules of Thumb"
10.4 Instrument Actuals
10.5 On-Line Signals

Module 11: ChemStation GC-AED Methods

11.1 What is a Method?
11.2 Creating a Method
11.3 Determining Peak Width
11.4 Running a Method

Module 12: ChemStation GC-AED Sequences

12.1 What is a Sequence?
12.2 Sequence Parameters
12.3 Sequence Table
12.4 Running a Sequence or Partial Sequence

Module 13: GC-AED Data Analysis

13.1 What is Data Analysis?
13.2 GC-AED Data Analysis Overview
13.3 Integration
13.4 Calibration and Quantitation
13.5 Spectral Manipulations

Module 14: GC-AED Pre-Flight Checklist

14.1 GC Setup and Ready
14.2 AED Setup and Ready
14.4 ChemStation Setup and Ready

Module 15: GC-AED Method Development

15.1 Comparison of GC Detectors
15.2 AED Precision and Accuracy
15.3 Determining Amount to Inject
15.4 Element Ratios and Linearity
15.5 Interelement Interferences
15.6 Choosing Columns
15.7 Injection Techniques

Module 16: GC-AED Maintenance

16.1 Preventative Maintenance Schedule
16.2 Discharge Tube
16.3 Spectrometer Lens
16.4 GC Maintenance
16.5 Setting AED Make-up and Purge Flow
16.6 Setting Reagent Gas Pressures
16.7 Evaluating UV Throughput

Module 17: Important Tips and Tricks

Laboratory Exercises

Exercise 1: Outline Application
Exercise 2: GC-AED Data Files
Exercise 3: GC-AED Instrument Components
Exercise 4: ChemStation Menu Tour
Exercise 5: AED Signal Generation
Exercise 6: Suppression and Back Amount
Exercise 7: Correct Data Rate
Exercise 8: Create a Method
Exercise 9: Create a Sequence
Exercise 10: Integration Techniques
Exercise 11: CIC Quantitation
Exercise 12: Spectral Manipulation
Exercise 13: Amount to Inject